- Recreated NIR Calibration equations with two pools
- High WSP pool (su1 and se)
- Low WSP pool
- With Field inbreds (sh2, sh2i, field, aeduwx)
- No Field inbreds (sh2 and sh2i only in both calibration and prediction)
- Preliminary Statistical Analysis
- Heritability
- Linear Modeling
- GWAS with GAPIT
4/9/2021
Carbohydrate Research Analysis Update
Equation Quality
| WF All Wetlab Samples | WF Validation Wetlab Samples | WF Validation Wetlab and Zystro Samples | NF All Wetlab Samples | NF Validaion Wetlab Samples | NF Validation Wetlab and Zystro Samples | |
|---|---|---|---|---|---|---|
| Fructose | 0.494 | 0.771 | 0.622 | 0.498 | 0.824 | 0.615 |
| Glucose | 0.516 | 0.521 | 0.660 | 0.531 | 0.644 | 0.635 |
| Sucrose | 0.659 | 0.809 | 0.542 | 0.737 | 0.937 | 0.598 |
| Total Sugar | 0.706 | 0.834 | 0.790 | 0.795 | 0.947 | 0.841 |
| Starch | 0.609 | 0.845 | 0.619 | 0.689 | 0.735 | 0.322 |
| Total Polysaccharide | 0.612 | 0.896 | 0.291 | 0.688 | 0.907 | 0.329 |
| WSP | 0.703 | 0.867 | 0.690 | 0.677 | 0.915 | 0.626 |
| Average R^2 | 0.614 | 0.792 | 0.602 | 0.659 | 0.844 | 0.567 |
Total Wetlab Samples
Only WSMDP Validation Samples
WSMDP and Zystro Validation Samples
Total Wetlab Samples
Only WSMDP Validation Samples
WSMDP and Zystro Validation Samples
WF Group: Cleaned Predicted Carbohydrates
WithFieldCarb
NF Group: Cleaned Predicted Carbohydrates
WithFieldCarb
WF Group: Sugars By Endosperm
Sugars by Endosperm
WF Group: Sugars By Endosperm
Starch by Endosperm
Trait Quality and Heritability
| Carbohydrate | Mean(%)+/-SD | Range(%) | h^2 |
|---|---|---|---|
| Starch | 14.55 +/- 7.51 | -3.17-56.95 | 0.89 |
| Total.Polysaccharides | 27.07 +/- 8.66 | 0-54.35 | 0.94 |
| WSP | 12.83 +/- 8.08 | -7.51-37.25 | 0.94 |
| Glucose | 4.98 +/- 1.64 | 0.35-13.85 | 0.92 |
| Fructose | 5.11 +/- 1.85 | 0.37-11.41 | 0.92 |
| Sucrose | 11.8 +/- 6.73 | -2.55-36.71 | 0.96 |
| Total.Sugar | 21.78 +/- 9.84 | -1.2-52.84 | 0.96 |
Linear Model
Starch by Endosperm
WF Group: GAPIT
Total Poly Genomewise
WF Group: GAPIT
Total Poly Genomewise
WF Group: GAPIT