4/9/2021

Carbohydrate Research Analysis Update

  • Recreated NIR Calibration equations with two pools
    • High WSP pool (su1 and se)
    • Low WSP pool
      • With Field inbreds (sh2, sh2i, field, aeduwx)
      • No Field inbreds (sh2 and sh2i only in both calibration and prediction)
  • Preliminary Statistical Analysis
    • Heritability
    • Linear Modeling
  • GWAS with GAPIT

Equation Quality

WF All Wetlab Samples WF Validation Wetlab Samples WF Validation Wetlab and Zystro Samples NF All Wetlab Samples NF Validaion Wetlab Samples NF Validation Wetlab and Zystro Samples
Fructose 0.494 0.771 0.622 0.498 0.824 0.615
Glucose 0.516 0.521 0.660 0.531 0.644 0.635
Sucrose 0.659 0.809 0.542 0.737 0.937 0.598
Total Sugar 0.706 0.834 0.790 0.795 0.947 0.841
Starch 0.609 0.845 0.619 0.689 0.735 0.322
Total Polysaccharide 0.612 0.896 0.291 0.688 0.907 0.329
WSP 0.703 0.867 0.690 0.677 0.915 0.626
Average R^2 0.614 0.792 0.602 0.659 0.844 0.567

Total Wetlab Samples

Only WSMDP Validation Samples

WSMDP and Zystro Validation Samples

Total Wetlab Samples

Only WSMDP Validation Samples

WSMDP and Zystro Validation Samples

WF Group: Cleaned Predicted Carbohydrates

WithFieldCarb

NF Group: Cleaned Predicted Carbohydrates

WithFieldCarb

WF Group: Sugars By Endosperm

Sugars by Endosperm

WF Group: Sugars By Endosperm

Starch by Endosperm

Trait Quality and Heritability

Carbohydrate Mean(%)+/-SD Range(%) h^2
Starch 14.55 +/- 7.51 -3.17-56.95 0.89
Total.Polysaccharides 27.07 +/- 8.66 0-54.35 0.94
WSP 12.83 +/- 8.08 -7.51-37.25 0.94
Glucose 4.98 +/- 1.64 0.35-13.85 0.92
Fructose 5.11 +/- 1.85 0.37-11.41 0.92
Sucrose 11.8 +/- 6.73 -2.55-36.71 0.96
Total.Sugar 21.78 +/- 9.84 -1.2-52.84 0.96

Linear Model

Starch by Endosperm

WF Group: GAPIT

Total Poly Genomewise

Total Poly Genomewise

WF Group: GAPIT

Total Poly Genomewise

Total Poly Genomewise

WF Group: GAPIT

some text

WF Group: GAPIT

caption

caption

WF Group: GAPIT

Glucose

WF Group: GAPIT

WF Group: GAPIT

Sucrose

WF Group: GAPIT

Total Sugar

HWSP Group: GAPIT

WSP

HWSP Group: GAPIT

WSP

HWSP Group: GAPIT

WSP

HWSP Group: GAPIT

WSP

HWSP Group: GAPIT

WSP

HWSP Group: GAPIT

WSP

HWSP Group: GAPIT

WSP