4/9/2021
Carbohydrate Research Analysis Update
- Recreated NIR Calibration equations with two pools
- High WSP pool (su1 and se)
- Low WSP pool
- With Field inbreds (sh2, sh2i, field, aeduwx)
- No Field inbreds (sh2 and sh2i only in both calibration and prediction)
- Preliminary Statistical Analysis
- Heritability
- Linear Modeling
- GWAS with GAPIT
Equation Quality
|
|
WF All Wetlab Samples
|
WF Validation Wetlab Samples
|
WF Validation Wetlab and Zystro Samples
|
NF All Wetlab Samples
|
NF Validaion Wetlab Samples
|
NF Validation Wetlab and Zystro Samples
|
|
Fructose
|
0.494
|
0.771
|
0.622
|
0.498
|
0.824
|
0.615
|
|
Glucose
|
0.516
|
0.521
|
0.660
|
0.531
|
0.644
|
0.635
|
|
Sucrose
|
0.659
|
0.809
|
0.542
|
0.737
|
0.937
|
0.598
|
|
Total Sugar
|
0.706
|
0.834
|
0.790
|
0.795
|
0.947
|
0.841
|
|
Starch
|
0.609
|
0.845
|
0.619
|
0.689
|
0.735
|
0.322
|
|
Total Polysaccharide
|
0.612
|
0.896
|
0.291
|
0.688
|
0.907
|
0.329
|
|
WSP
|
0.703
|
0.867
|
0.690
|
0.677
|
0.915
|
0.626
|
|
Average R^2
|
0.614
|
0.792
|
0.602
|
0.659
|
0.844
|
0.567
|
Total Wetlab Samples
Only WSMDP Validation Samples
WSMDP and Zystro Validation Samples
Total Wetlab Samples
Only WSMDP Validation Samples
WSMDP and Zystro Validation Samples
WF Group: Cleaned Predicted Carbohydrates

WithFieldCarb
NF Group: Cleaned Predicted Carbohydrates

WithFieldCarb
WF Group: Sugars By Endosperm

Sugars by Endosperm
WF Group: Sugars By Endosperm

Starch by Endosperm
Trait Quality and Heritability
|
Carbohydrate
|
Mean(%)+/-SD
|
Range(%)
|
h^2
|
|
Starch
|
14.55 +/- 7.51
|
-3.17-56.95
|
0.89
|
|
Total.Polysaccharides
|
27.07 +/- 8.66
|
0-54.35
|
0.94
|
|
WSP
|
12.83 +/- 8.08
|
-7.51-37.25
|
0.94
|
|
Glucose
|
4.98 +/- 1.64
|
0.35-13.85
|
0.92
|
|
Fructose
|
5.11 +/- 1.85
|
0.37-11.41
|
0.92
|
|
Sucrose
|
11.8 +/- 6.73
|
-2.55-36.71
|
0.96
|
|
Total.Sugar
|
21.78 +/- 9.84
|
-1.2-52.84
|
0.96
|
Linear Model

Starch by Endosperm
WF Group: GAPIT
WF Group: GAPIT
WF Group: GAPIT
WF Group: GAPIT